Monday, March 09, 2009

My cells are blue!!! :D

And that is absolutely good news (means at least a part of my experiment worked)!!! :D (Thank you, for all the 'crossing' you all did for me!) I was so elated that I did a little dance around the lab! Hee... And the Prof was really happy to see that under the microscope too. That means I can continue with more experiments. It's so nice to get some (positive) results. Especially since I've repeated this experiment twice before with not-too-heartening results (although each time was slightly better than the next). And it's with some pride that I can say I'd suggested the changes to the protocol after reading a paper doing similar viral transfection. (A bit of self-consolation that I am not so stupid after all. hee.)
Am hoping that later when we look at part II (plasmid transfection) of the experiments which I finished over the weekend, it will also give us some hope for future work.
I have less than a month left in this lab, so hope that the experiments will go on smoothly (and that I don't have to come back every weekend till then)...

Update: The plasmid transfections did not seem to work (i.e. no obvious differences in fluorescence between transfected and control cells). :( But since we are trying out a new protocol, probably it still needs to be refined and there's yet hope... Let's see...
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