Monday, March 09, 2009
My cells are blue!!! :D
And that is absolutely good news (means at least a part of my experiment worked)!!! :D (Thank you, for all the 'crossing' you all did for me!) I was so elated that I did a little dance around the lab! Hee... And the Prof was really happy to see that under the microscope too. That means I can continue with more experiments. It's so nice to get some (positive) results. Especially since I've repeated this experiment twice before with not-too-heartening results (although each time was slightly better than the next). And it's with some pride that I can say I'd suggested the changes to the protocol after reading a paper doing similar viral transfection. (A bit of self-consolation that I am not so stupid after all. hee.)
Am hoping that later when we look at part II (plasmid transfection) of the experiments which I finished over the weekend, it will also give us some hope for future work.
I have less than a month left in this lab, so hope that the experiments will go on smoothly (and that I don't have to come back every weekend till then)...
Update: The plasmid transfections did not seem to work (i.e. no obvious differences in fluorescence between transfected and control cells). :( But since we are trying out a new protocol, probably it still needs to be refined and there's yet hope... Let's see...
Am hoping that later when we look at part II (plasmid transfection) of the experiments which I finished over the weekend, it will also give us some hope for future work.
I have less than a month left in this lab, so hope that the experiments will go on smoothly (and that I don't have to come back every weekend till then)...
Update: The plasmid transfections did not seem to work (i.e. no obvious differences in fluorescence between transfected and control cells). :( But since we are trying out a new protocol, probably it still needs to be refined and there's yet hope... Let's see...
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